31 May 2007
Rapd typing of methicillin-resistant Staphylococcus aureus: A 7-year experience in a Polish hospital
Julianna Kurlenda, Mariusz Grinholc, Krzysztof Jasek, Grzegorz WegrzynMed Sci Monit 2007; 13(6): MT13-18 :: ID: 484351
Abstract
Background: Methicillin-resistant strains of Staphylococcus aureus (MRSA) are important etiological factors responsible for healthcare-associated infections. The aim of this study was to evaluate the epidemic and to discriminate all of the involved strains isolated at the Provincial Hospital in Gdańsk and, on this basis, perform an epidemiological investigation using the random amplification of polymorphic DNA PCR (RAPD) with the primer AP-7.
Material/Methods: Two hundred and thirty-four isolates of Staphylococcus aureus were typed to evaluate a seven-year epidemic of MRSA at the hospital. All isolates were recovered from infection and carriage sites of patients. Numerous strains were isolated from patients suffering from generalized infections, bacteremia, and endoprosthesis-related infections.
Results: Using the RAPD PCR method, the collection of 234 MRSA strains was divided into 10 groups, one of which was the most common (81%). No correlation between the S. aureus clones determined in this way and hospital department or the type of infection was found.
Conclusions: It was confirmed that MRSA infections may be easily spread throughout a hospital and that the introduction of a surveillance program implemented with control procedures is sufficient to restrict serious problems such as an MRSA epidemic.
Keywords: Hospitals, Bacterial Typing Techniques, Methicillin Resistance - genetics, Poland, Polymerase Chain Reaction, Random Amplified Polymorphic DNA Technique - methods, Staphylococcus aureus - isolation & purification, Bacterial Typing Techniques, Hospitals, Methicillin Resistance - genetics, Poland, Polymerase Chain Reaction, Random Amplified Polymorphic DNA Technique - methods, Staphylococcus aureus - isolation & purification
Editorial
01 May 2024 : Editorial
Editorial: First Regulatory Approval for Adoptive Cell Therapy with Autologous Tumor-Infiltrating Lymphocytes (TILs) – Lifileucel (Amtagvi)DOI: 10.12659/MSM.944927
Med Sci Monit 2024; 30:e944927
In Press
15 Mar 2024 : Clinical Research
Evaluation of an Optimized Workflow for the Radiofrequency Catheter Ablation of Paroxysmal Atrial FibrillationMed Sci Monit In Press; DOI: 10.12659/MSM.943526
09 May 2024 : Review article
A Review of the Current Status of Disease-Modifying Therapies and Prevention of Alzheimer’s DiseaseMed Sci Monit In Press; DOI: 10.12659/MSM.945091
09 Apr 2024 : Clinical Research
Correlation between Thalamocortical Tract and Default Mode Network with Consciousness Levels in Hypoxic-Isc...Med Sci Monit In Press; DOI: 10.12659/MSM.943802
19 Apr 2024 : Clinical Research
Comparative Analysis of Postoperative Sagittal Balance in Expansive Open-Door Laminoplasty versus Laminecto...Med Sci Monit In Press; DOI: 10.12659/MSM.943057
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952